The second stage began with a BLAST search of sliced sequences within a negative group database, to reveal ones which had no similarities with the negative group genomes. atrosepticum type strain into a set of short sequences of 100 bp, overlapping by 90 bp.

The first (preparation) stage entailed collecting genomes of positive and negative groups, creating BLAST databases for the collected sequences and splitting the target genome of the P. However, the development of novel assays, characterized by improved specificity on the level of species, is still needed for both pathogen monitoring and taxonomic studies of this group of organisms. Additionally, the phytopathological practice may not necessarily require fine differentiation of SRP because the symptoms of the disease are mostly similar for all species. This inconsistency is mostly explained by recent changes at the taxonomic level, assigning some previously sequenced strains to different species. peruviense, and conversely, are not completely complementary to the target sequences in the genomes of some Pat strains. can amplify target sequences in the genomes of P. peruviense genomes potentially resulting in false-positive amplifications. For instance, the primers offered by De Boer and Ward are complimentary to the corresponding sequences in P. A BLAST analysis performed at mid-2020 and based on both complete and draft genome sequences retrieved from GenBank showed a high risk of false-positive results when using existing sets of Pat-specific primers.

Geneious prime dow